PathScan ® Sandwich ELISA Lysis Buffer (1X) from Cell Signaling Technology

Product Specs
ItemPathScan ® Sandwich ELISA Lysis Buffer (1X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7018S
Quantity30 ml
Research AreaRelated Products
ApplicationsELISA
Clone1X

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The Rac inhibitor HV-107 as a potential therapeutic for metastatic breast cancerMolecular MedicineJune 14, 2023Grace Velez Crespo, Jescelica Ortiz, Eliud Hernández O’Farrill, Cornelis P. Vlaar, Mikhail Inyushin, Yuriy Kucheryavykh, Lilia KucheryavykhLincoln, NE, USA). Western blot analysis Cells were lysed in 1X PathScan Sandwich ELISA lysis buffer (#7018, Cell Signalling, Denver, MA, USA) supplemented with a 1 mM protease inhibitor cocktail. Clarified cell

(2) Tau Regulates Glioblastoma Progression, 3D Cell Organization, Growth and Migration via the PI3K-AKT AxisCancersNovember 19, 2021Pagano A, Breuzard G, Parat F, Tchoghandjian A, Figarella-Branger D, De Bessa TC, Garrouste F, Douence A, Barbier P, Kovacic H.least three times. 2.7. Western Blot Analysis Cells or MCS were lysed in Pathscan lysis buffer (#7018, Cell Signaling Technology, Danvers, MA, USA): 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM disodium EDTA,

(3) Bioactive recombinant human oncostatin M for NMR-based screening in drug discoveryScientific reportsAugust 10, 2021Mass OA, Tuccinardi J, Woodbury L, Wolf CL, Grantham B, Holdaway K, Pu X, King MD, Warner DL, Jorcyk CL, et al.cell lysates were collected with 1X Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA; Cat# 7018). Lysates were run on an SDS-PAGE gel and transferred onto a nitrocellulose membrane via semi-dry transfer.

(4) Long-chain polyphosphates impair SARS-CoV-2 infection and replicationScience SignalingJuly 7, 2021Veronica Ferrucci et al.the manufacturer’s instructions. Briefly, the cells were lysed in 30 μl of PathScan lysis buffer (no. 7018, Cell Signaling Technology). Cell lysates (4 μg) were mixed with substrate solution (N-acetyl-Asp-Glu-Val-Asp-7-amino-4-methylcoumarin;

(5) Downregulation of long non‑coding RNA GACAT1 suppresses proliferation and induces apoptosis of NSCLC cells by sponging microRNA‑422aInternational Journal of Molecular MedicineDecember 22, 2020Youqing Zhong, Hui Lin, Qi Li, Chang Liu, Lei ZhongsiRNA-GACAT1. After transfection for 48 h, cells were harvested and lysed with 50 µl lysis buffer (cat. no. 7018; Cell Signaling Technology, Inc.). A total of 25 µl lysate solution was mixed with 200 µl substrate

(6) Insight into the Interactome of Intramitochondrial PKA Using Biotinylation-Proximity LabelingInternational Journal of Molecular SciencesNovember 5, 2020Yasmine Ould Amer, Etienne Hebert-Chatelainthe indicated plasmids were resuspended in lysis buffer (PathScan® Sandwich ELISA 1X, Cell Signaling #7018) supplemented with 1% protease inhibitor cocktail, 2 mM sodium orthovanadate and 1 mM sodium fluoride.

(7) Polarized human cholangiocytes release distinct populations of apical and basolateral small extracellular vesiclesMolecular Biology of the CellOctober 15, 2020Brian A. Davies, Leslie O. Morton, John R. Jefferson, Cody N. Rozeveld, Luke C. Doskey, Nicholas F. LaRusso, David J. Katzmannwere washed in cold PBS and lysed in PathScan Sandwich ELISA Lysis Buffer (Cell Signaling Technologies 7018) with the addition of Complete EDTA-free Protease Inhibitor Cocktail Tablet (Roche 04693132001), PhosSTOP

(8) ABTB2 Regulatory Variant as Predictor of Epirubicin-Based Neoadjuvant Chemotherapy in Luminal A Breast CancerFrontiers in OncologySeptember 25, 2020Yajie Gong et al.were rinsed with ice-cold phosphate-buffered saline and subsequently cell lysis buffer was added (#7018, Cell Signaling Technology, Danvers, MA, USA). Caspase activity assay (Caspase-3 Activity Assay Kit

(9) Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivoJournal of extracellular vesiclesAugust 7, 2020Elashiry M, Elashiry MM, Elsayed R, Rajendran M, Auersvald C, Zeitoun R, Rashid MH, Ara R, Meghil MM, Liu Y, et al.computationally predicted miRNA-mRNA target relationships [37]. ELISA EXO lysed with ELISA lysis buffer (#7018, Cell signaling) or intact were suspended in PBS and analysed according to the manufacturer, using ELISA

(10) Evolution of tumor cells during AsiDNA treatment results in energy exhaustion, decrease in responsiveness to signal, and higher sensitivity to the drugEvolutionary ApplicationsAugust 1, 2020Pierre‐Marie Girard, Nathalie Berthault, Maria Kozlac, Sofia Ferreira, Wael Jdey, Srividya Bhaskara, Sergey Alekseev, Frederic Thomas, Marie Dutreixcell scraper and boiled for 10 min in PathScan Sandwich ELISA Lysis Buffer (Cell Signaling Technology, 7018) and 1 mM PMSF (phenylmethanesulfonyl fluoride; Sigma, P7626) after treatment. Quantification of total

(11) Treatment with catalpol protects against cisplatin‑induced renal injury through Nrf2 and NF‑κB signaling pathwaysExperimental and Therapeutic MedicineJuly 29, 2020Jun Zhang, Li Liu, Furong Li, Zongqian Wang, Jinghong Zhaowere homogenized with 1 ml PBS (pH 7.4, 100 mM) and PathScan® Sandwich ELISA lysis buffer (cat. no. 7018; Cell Signaling Technology, Inc.) was used to lyse the tissues before centrifugation at 3,660 x g for

(12) Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal InjuryEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Huang YS, Wang X, Feng Z, Cui H, Zhu Z, Xia C, Han X, Liu WJ, Liu YN.Assay of Cells Cells were treated for 72 h and then lysed in PathScan Sandwich ELISA lysis buffer (1×; 7018, Cell Signaling Technology). Cell lysate was added to the assay plates containing the substrate solution,

(13) Knockdown Of CCDC132 Attenuates Gastric Cancer Cells Proliferation And Tumorigenesis By Facilitating DNA Damage SignalingCancer Management and ResearchNovember 12, 2019Xiaowu Xu, Weilang Cao, Wei Sun, Zhaohong Wang, Hui Chen, Zhiqiang Zheng, Xiaomin Yang16-Nov-2021 For personal use only. Xu et al prepared by PathScan® Sandwich ELISA Lysis Buffer (cat. no. 7018; Cell Signaling, USA). Protein concentrations were determined using the BCA Protein Assay Kit (cat.

(14) Mutant IDH sensitizes gliomas to endoplasmic reticulum stress and triggers apoptosis via microRNA183-mediated inhibition of Semaphorin 3ECancer ResearchOctober 1, 2019Ying Zhang et al.to determine the D2HG concentration in each sample. Caspase-3/7 activity assay Cells were lysed (#7018; Cell Signaling) and, 5-10 μg of the lysate was mixed with 150 μl protease assay buffer containing 20

(15) Siglec-7 on peripheral blood eosinophils: Surface expression and functionAllergyJuly 1, 2019Fanny Legrand, Nadine Landolina, Ilan Zaffran, Robert O. Emeh, Elizabeth Chen, Amy D. Klion, Francesca Levi-Schafferice-cold PBS to the pellet followed by centrifugation. Cell pellets were lysed using a cell lysis buffer (#7018 Cell Signaling Technology, Danvers, MA) supplemented with protease inhibitor cocktail (1:100, Sigma-Aldrich,

(16) Unmasking a new prognostic marker and therapeutic target from the GDNF-RET/PIT1/p14ARF/p53 pathway in acromegalyEBioMedicineMay 1, 2019Chenlo M, Rodriguez-Gomez IA, Serramito R, Garcia-Rendueles AR, Villar-Taibo R, Fernandez-Rodriguez E, Perez-Romero S, Suarez-Fariña M, Garcia-Allut A, Cabezas-Agricola JM, et al.hour. Cells were washed with ice-cold PBS and lysed with 30 microL of ice-cold Cell Lysis buffer (#7018, Cell Signaling Technology, USA). MicroBCA Protein Assay (23,235 Thermo IL USA) was used to measure

(17) HIGH expression of OSM and IL-6 are associated with decreased breast cancer survival: synergistic induction of IL-6 secretion by OSM and IL-1βOncotargetMarch 12, 2019Tawara K, Scott H, Emathinger J, Wolf C, LaJoie D, Hedeen D, Bond L, Montgomery P, Jorcyk C.cells per well in a 24-well plate. Cells were lysed with Cell Signaling PathScan® Lysis buffer (Cat# 7018) using the manufacturer’s instructions. The lysates were then used on a Dynabeads® Protein A Immunoprecipitation Kit

(18) Effects of UPF1 expression on EMT process by targeting E‑cadherin, N‑cadherin, Vimentin and Twist in a hepatocellular carcinoma cell lineMolecular Medicine ReportsJanuary 10, 2019Yawei Li, Tiantian Zhang, Shukui Qin, Rui Wang, Yumei Li, Zhengguang Zhou, Yufo Chen, Qiong Wu, Fang Supresence of UPF1 overexpression. A total of 2×105 cells were lysed using cell lysis buffer (cat. no. 7018; Cell Signaling Technology, Inc.) on ice, and then incubated with a slide for 2 h at room temperature

(19) Knockdown of EIF3C promotes human U-2OS cells apoptosis through increased CASP3/7 and Chk1/2 by upregulating SAPK/JNKOncoTargets and therapyJanuary 1, 2019Gao W, Hu Y, Zhang Z, Du G, Yin L, Yin Z.cells transfected with shEIF3C or shCtrl lentivirus were lysed with 1X Cell Lysis Buffer (catalog no 7018; Cell Signaling Technology). After collection of whole-cell lysates, protein concentration was determined

(20) Oleoylethanolamide modulates glucagon-like peptide-1 receptor agonist signaling and enhances exendin-4-mediated weight loss in obese miceAmerican Journal of Physiology-Regulatory, Integrative and Comparative PhysiologyOctober 1, 2018Jacob D. Brown, Danielle McAnally, Jennifer E. Ayala, Melissa A. Burmeister, Camilo Morfa, Layton Smith, Julio E. Ayalastored at ⫺80°C until analysis. Cell homogenates were processed by scraping in cell lysis buffer (no. 7018; Cell Signaling Technology) containing protease and phosphatase inhibitors. Supernatant of combined

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PathScan ® Sandwich ELISA Lysis Buffer (1X) from Cell Signaling Technology

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PathScan ® Sandwich ELISA Lysis Buffer (1X) from Cell Signaling Technology

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